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human aortic vascular smooth muscle cells  (ATCC)


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    ATCC human aortic vascular smooth muscle cells
    Human Aortic Vascular Smooth Muscle Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+aortic+vascular+smooth+muscle+cells/T%2FG+HA-VSMC/pmc13218890-117-7-17
    Average 96 stars, based on 1111 article reviews
    human aortic vascular smooth muscle cells - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: S1PR2 Mediates Smooth Muscle Cell Proliferation and Endothelial Cell Permeability via Akt/mTOR and RhoA/ROCK1 in Atherosclerosis.
    Article Snippet: Atherosclerosis is a complex disease involving a series of interrelated events, among which endothelial cell dysfunction and vascular smooth muscle cell proliferation play pivotal roles in the early formation of atherosclerotic plaques.. Sphingosine 1‐phosphate, a product of sphingomyelin metabolism, serves as a signaling molecule implicated in the pathogenesis of atherosclerosis and other conditions by binding to sphingosine 1‐phosphate receptors (S1PRs).. However, the precise mechanism by which S1PRs influence atherosclerosis remains incompletely understood.

    Article Title: Physiologically relevant platform for an advanced in vitro model of the vascular wall: focus on in situ fabrication and mechanical maturation
    Article Snippet: .. Human aortic vascular smooth muscle cells (T/G HAVSMC, ATCC® CRL1999TM, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco), 1% Penicillin–Streptomycin solution (Pen-Strep, Gibco), 5 μg/mL human insulin (Santa Cruz Biotechnology, Dallas, TX, USA), 2 ng/mL Fibroblast Growth Factor-basic (FGFb, Gibco), and 0.5 ng/mL Epidermal Growth Factor (EGF, Invitrogen, Thermo Fisher Scientific). .. Neonatal human dermal fibroblasts (HDFn, C0045C, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) were cultured in DMEM supplemented with 10% FBS, 1% Pen-Strep, 2 ng/mL FGFb, 0.5 ng/mL EGF, and 1 μg/mL hydrocortisone (Sigma, St. Louis, MO, USA).

    Article Title: Hsa_circRNA_0008028 Deficiency Ameliorates High Glucose-Induced Proliferation, Calcification, and Autophagy of Vascular Smooth Muscle Cells via miR-182-5p/TRIB3 Axis
    Article Snippet: .. Human aortic vascular smooth muscle cells (ATCC, CRL-1999) were cultured in the Dulbecco's Modified Eagle's Medium (DMEM, HyClone, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA), penicillin (100 U/mL, Beyotime, China), and streptomycin (0.1 mg/mL, Beyotime, China) in a humidified atmosphere with 5% CO 2 at 37°C. ..

    In Vitro:

    Article Title: Integrative multivariate genomic analysis reveals shared genetic determinants and druggable targets for vascular calcification
    Article Snippet: .. To establish an in vitro calcification model, human aortic vascular smooth muscle cells (VSMCs; CRL-1999) obtained from ATCC (Manassas, VA, United States) were exposed to a phosphate-enriched environment containing 3 mM inorganic phosphate (Pi) for 10 days, with media replacement performed every 48 h. Prior to induction, cells at passages 3–8 were maintained under standard conditions in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific, United States) supplemented with 10% fetal bovine serum (FBS; Gibco, United States). .. For routine culture, a reduced-serum maintenance medium was used, consisting of DMEM with 2% FBS and antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin; Thermo Fisher Scientific, United States).

    Modification:

    Article Title: Integrative multivariate genomic analysis reveals shared genetic determinants and druggable targets for vascular calcification
    Article Snippet: .. To establish an in vitro calcification model, human aortic vascular smooth muscle cells (VSMCs; CRL-1999) obtained from ATCC (Manassas, VA, United States) were exposed to a phosphate-enriched environment containing 3 mM inorganic phosphate (Pi) for 10 days, with media replacement performed every 48 h. Prior to induction, cells at passages 3–8 were maintained under standard conditions in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific, United States) supplemented with 10% fetal bovine serum (FBS; Gibco, United States). .. For routine culture, a reduced-serum maintenance medium was used, consisting of DMEM with 2% FBS and antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin; Thermo Fisher Scientific, United States).

    Article Title: Physiologically relevant platform for an advanced in vitro model of the vascular wall: focus on in situ fabrication and mechanical maturation
    Article Snippet: .. Human aortic vascular smooth muscle cells (T/G HAVSMC, ATCC® CRL1999TM, Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco), 1% Penicillin–Streptomycin solution (Pen-Strep, Gibco), 5 μg/mL human insulin (Santa Cruz Biotechnology, Dallas, TX, USA), 2 ng/mL Fibroblast Growth Factor-basic (FGFb, Gibco), and 0.5 ng/mL Epidermal Growth Factor (EGF, Invitrogen, Thermo Fisher Scientific). .. Neonatal human dermal fibroblasts (HDFn, C0045C, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) were cultured in DMEM supplemented with 10% FBS, 1% Pen-Strep, 2 ng/mL FGFb, 0.5 ng/mL EGF, and 1 μg/mL hydrocortisone (Sigma, St. Louis, MO, USA).

    Article Title: Hsa_circRNA_0008028 Deficiency Ameliorates High Glucose-Induced Proliferation, Calcification, and Autophagy of Vascular Smooth Muscle Cells via miR-182-5p/TRIB3 Axis
    Article Snippet: .. Human aortic vascular smooth muscle cells (ATCC, CRL-1999) were cultured in the Dulbecco's Modified Eagle's Medium (DMEM, HyClone, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA), penicillin (100 U/mL, Beyotime, China), and streptomycin (0.1 mg/mL, Beyotime, China) in a humidified atmosphere with 5% CO 2 at 37°C. ..



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    The effect of TCC on the biological function of vascular smooth muscle cells (VSMCs). (A) Apoptosis experiment. (B) Statistical chart of apoptosis experiment data. (C) CCK-8 experiment determines cell viability. (D) Migration experiment. (E) Migration Capability Analysis. # p < 0.05 vs. TCC, * p < 0.05 vs. Control.

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: Research and development of novel embolization materials and study on their feasibility of preventing T2EL after EVAR abdominal aortic aneurysm

    doi: 10.3389/fcvm.2026.1740816

    Figure Lengend Snippet: The effect of TCC on the biological function of vascular smooth muscle cells (VSMCs). (A) Apoptosis experiment. (B) Statistical chart of apoptosis experiment data. (C) CCK-8 experiment determines cell viability. (D) Migration experiment. (E) Migration Capability Analysis. # p < 0.05 vs. TCC, * p < 0.05 vs. Control.

    Article Snippet: Human aortic vascular smooth muscle cells (VSMCs) and human aortic endothelial cells (VECs) were obtained from Wuhan Procell Life Science & Technology Co., Ltd. All cells were primary cells and used between passages 3–8.

    Techniques: CCK-8 Assay, Migration, Control

    The effect of TCC on the biological function of vascular smooth muscle cells (VECs). (A) Apoptosis experiment. (B) Statistical chart of apoptosis experiment data. (C) CCK-8 experiment determines cell viability. (D) Migration experiment. (E) Migration Capability Analysis. # p < 0.05 vs. TCC, * p < 0.05 vs. Control.

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: Research and development of novel embolization materials and study on their feasibility of preventing T2EL after EVAR abdominal aortic aneurysm

    doi: 10.3389/fcvm.2026.1740816

    Figure Lengend Snippet: The effect of TCC on the biological function of vascular smooth muscle cells (VECs). (A) Apoptosis experiment. (B) Statistical chart of apoptosis experiment data. (C) CCK-8 experiment determines cell viability. (D) Migration experiment. (E) Migration Capability Analysis. # p < 0.05 vs. TCC, * p < 0.05 vs. Control.

    Article Snippet: Human aortic vascular smooth muscle cells (VSMCs) and human aortic endothelial cells (VECs) were obtained from Wuhan Procell Life Science & Technology Co., Ltd. All cells were primary cells and used between passages 3–8.

    Techniques: CCK-8 Assay, Migration, Control